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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 11.21
Human Site: T1274 Identified Species: 17.62
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 T1274 K R E P G T K T K K Q T T L A
Chimpanzee Pan troglodytes XP_516332 1634 184611 V1322 K K E P G T R V R K T P T S S
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 V1308 K K E P G T R V R K T P T S S
Dog Lupus familis XP_537646 1532 174610 T1274 K R E P G T K T K K Q T T L P
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 A1270 K Q K K E P G A K K Q T T L P
Rat Rattus norvegicus P41516 1526 173202 A1270 K R E P G T R A K K Q T T L P
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 G1304 K S D T G T K G K K Q N T L L
Chicken Gallus gallus O42130 1553 174974 T1278 K R K A E Q G T K R Q T T L P
Frog Xenopus laevis NP_001082502 1579 178601 D1286 P G T K A V K D K K Q A T L P
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 S1284 T K D K G S K S G K Q T T L T
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 N1191 R A E E Q G I N L K T A K A L
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 R1256 A A D A K T G R G P K K N V C
Sea Urchin Strong. purpuratus XP_783546 1448 163750 T1191 K T A S M L W T D D L N A F M
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 P1209 K R Q A P K K P A P K K T T K
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 D1173 Y Q E F L Q R D A E A R G G N
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 46.6 46.6 93.3 N.A. 46.6 80 N.A. 60 53.3 40 46.6 N.A. 13.3 N.A. 6.6 13.3
P-Site Similarity: 100 73.3 73.3 93.3 N.A. 60 86.6 N.A. 66.6 66.6 40 73.3 N.A. 20 N.A. 26.6 13.3
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 26.6 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 40 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 14 7 20 7 0 0 14 14 0 7 14 7 7 7 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 % C
% Asp: 0 0 20 0 0 0 0 14 7 7 0 0 0 0 0 % D
% Glu: 0 0 47 7 14 0 0 0 0 7 0 0 0 0 0 % E
% Phe: 0 0 0 7 0 0 0 0 0 0 0 0 0 7 0 % F
% Gly: 0 7 0 0 47 7 20 7 14 0 0 0 7 7 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 % I
% Lys: 67 20 14 20 7 7 40 0 47 67 14 14 7 0 7 % K
% Leu: 0 0 0 0 7 7 0 0 7 0 7 0 0 54 14 % L
% Met: 0 0 0 0 7 0 0 0 0 0 0 0 0 0 7 % M
% Asn: 0 0 0 0 0 0 0 7 0 0 0 14 7 0 7 % N
% Pro: 7 0 0 34 7 7 0 7 0 14 0 14 0 0 34 % P
% Gln: 0 14 7 0 7 14 0 0 0 0 54 0 0 0 0 % Q
% Arg: 7 34 0 0 0 0 27 7 14 7 0 7 0 0 0 % R
% Ser: 0 7 0 7 0 7 0 7 0 0 0 0 0 14 14 % S
% Thr: 7 7 7 7 0 47 0 27 0 0 20 40 74 7 7 % T
% Val: 0 0 0 0 0 7 0 14 0 0 0 0 0 7 0 % V
% Trp: 0 0 0 0 0 0 7 0 0 0 0 0 0 0 0 % W
% Tyr: 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _